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Fast Mutagenesis System

SKU: BTL-TB-MB-00326 | Brand: Transgen Biotek
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Product Description

Cat Number FM111-01
Category Molecular Biology Product
Pack Size 10 rxns
Description • Primers anneal to the DNA template, mutant strands are synthesized with 2×TransStart® FastPfu PCR SuperMix. • In vitro digestion of non-mutated parental plasmid (methylated plasmid) with DMT enzyme and in vivo degradation of non-mutated parental plasmid (methylated plasmid) with DMT Chemically Competent Cell, so as to efficiently select mutant clones. Features • Mutation sites on both primers to improve mutation efficiency. • Partially overlapping primers for exponential DNA amplification. • Fast (4 kb/min) and high fidelity ( 54-fold fidelity as compared to EasyTaq® DNA Polymerase) 2xTransStart® FastPfu PCR SuperMix for DNA amplification. • Double digestions (in vitro and in vivo) of parental plasmids to enhance mutation efficiency. Storage Competent Cell at -70°C for six months; others at -20°C for two years Shipping Dry ice (-70℃)
Storage -80°C
References 1 Yang F, Chen Y, Liu Q, et al. Dynamics and regulations of BimEL Ser65 and Thr112 phosphorylation in porcine granulosa cells during follicular atresia[J]. Cells, 2020.(IF 5.27) 2 Ke Y, Lv X, Fu X, et al. Poly (ADP-ribosyl) ation enhances HuR oligomerization and contributes to pro-inflammatory gene mRNA stabilization[J]. Cellular and Molecular Life Sciences, 2021.(IF 6.49) 3 Ouyang M, Li X, Zhang J, et al. Liquid-liquid phase transition drives intra-chloroplast cargo sorting[J]. Cell, 2020.(IF 66.85) 4 Song N, Qi Q, Cao R, et al. MAVS O-GlcNAcylation is essential for host antiviral immunity against lethal RNA viruses[J]. Cell reports, 2019.(IF 7.81) 5 Hu Z, He J, Chen K, et al. Molecular cloning and biochemical characterization of a new flavonoid glycosyltransferase from the aquatic plant lotus[J]. Biochemical and biophysical research communications, 2019.(IF 12.10) 6 You L, Shi J, Shen L, et al. Structural basis for transcription antitermination at bacterial intrinsic terminator[J]. Nature communications, 2019.(IF 11.80) 7 Liu M, Xu B, Ma Y, et al. Reversible covalent inhibitors suppress enterovirus 71 infection by targeting the 3C protease[J]. Antiviral Research, 2021.(IF 5.97) 8 Liu X, Liu Z, Wu Z, et al. Resurrection of endogenous retroviruses during aging reinforces senescence[J]. Cell, 2023.(IF 66.85) 9 Tian Y, Chen Z H, Wu P, et al. MIR497HG‐Derived miR‐195 and miR‐497 Mediate Tamoxifen Resistance via PI3K/AKT Signaling in Breast Cancer[J]. Advanced Science, 2023.(IF 17.52) 10 Wang K, Zhang Z, Hang J, et al. Microbial-host-isozyme analyses reveal microbial DPP4 as a potential antidiabetic target[J]. Science, 2023.(IF 56.90) 11 Shi C, Yang X, Hou Y, et al. USP15 promotes cGAS activation through deubiquitylation and liquid condensation[J]. Nucleic Acids Research, 2022.(IF 14.90) 12 Chen Y G, Li D S, Ling Y, et al. A cryptic plant terpene cyclase producing unconventional 18‐and 14‐membered macrocyclic C25 and C20 terpenoids with immunosuppressive activity[J]. Angewandte Chemie, 2021.(IF 15.33) 13 Huang K, Wu X X, Fang C L, et al. Pol IV and RDR2: a two-RNA-polymerase machine that produces double-stranded RNA[J]. Science, 2021.(IF 63.71)
Note The product is for research use only
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