Product Description
| Cat Number | E-CL-H0992_48T |
|---|---|
| Category | Chemiluminescence Assay Kit |
| Pack Size | 48T |
| Detection Method | Chemiluminescence |
| Research Field | Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Metabolism, Microbiology, Tags and Cell Markers |
| Sensitivity | 37.5 pg/mL |
| Detection Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Sample Type | Tissue homogenates,cell lysates and other biological fluids |
| Assay Length | 3 h 30 min |
| Uniprot | Q96EB6 |
| Target Synonyms | SIR2L1;NAD-dependent deacetylase sirtuin-1; |
| Test Principle | This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SIRT1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SIRT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SIRT1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human SIRT1. You can calculate the concentration of Human SIRT1 in the samples by comparing the RLU value of the samples to the standard curve. |
| Storage | 2-8℃/-20℃ |
| Standard Curve | View Document |
| Technical Manual | View Document |
| Note | The product is for research use only |
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