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Ethanol Colorimetric Assay Kit

SKU: BTL-EL-AK-02797 | Brand: Elabscience
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Product Description

Cat Number E-BC-K891-M_96T
Category Cell Metabolism Assay Kit
Pack Size 96T
Detection Method Colorimetric method
Research Field Liver and Renal Function
Sensitivity 0.27 μmol/mL
Detection Range 0.27–10.0 μmol/mL
Sample Type serum;plasma;wine
Assay Length 30 min
Reader Microplate reader
Test Principle Ethanol dehydrogenase can catalyze oxidative dehydrogenation of ethanol to acetaldehyde, and NAD+ is reduced to produce NADH. NADH, under the action of 1-mPMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, ethanol content can be quantified by measure the OD value at 450 nm.
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Note The product is for research use only
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