Cell Mitochondrial Complex II Activity Assay Kit
SKU: BTL-EL-AK-02744 |
Brand: Elabscience
Product Description
| Cat Number | E-BC-K835-M_48T |
|---|---|
| Category | Cell Metabolism Assay Kit |
| Pack Size | 48T |
| Detection Method | Colorimetric method |
| Research Field | Mitochondria Function |
| Sensitivity | 0.54 U/L |
| Detection Range | 0.54-17.66 U/L |
| Sample Type | cell |
| Assay Length | 30 min |
| Target Synonyms | succinate-coenzyme Q reductase |
| Reader | Microplate reader |
| Test Principle | Mitochondrial complex II, also known as succinate-coenzyme Q reductase, is widely found in the mitochondria of animals, plants, microorganisms and cultured cells. It catalyzes the oxidation of succinic acid in the TCA cycle to fumaric acid, which converts the ubiquinone to a reduced form through the basic unit structures of the complex such as iron-sulfur proteins. Coenzyme Q, the catalytic product of mitochondrial complex II, can further reduce 2, 6-dichloroindoxol, which has a characteristic absorption peak at 600 nm. Therefore, the activity of mitochondrial complex II can be quantified by measure the change OD value at 600 nm. |
| Storage | This product can be stored at 2~8°C/-20°C~-80°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Technical Manual | View Document |
| Msds | View Document |
| Product Url | View Document |
| Note | The product is for research use only |
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