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Total Oxidant Status (TOS) Colorimetric Assay Kit

SKU: BTL-EL-AK-02717 | Brand: Elabscience
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Product Description

Cat Number E-BC-K802-M_500Assays
Category Cell Metabolism Assay Kit
Pack Size 500Assays
Detection Method Colorimetric method
Research Field Oxidative stress
Sensitivity 2.5 μmol H2O2 Equiv./L
Detection Range 2.5-100 μmol H2O2 Equiv./L
Sample Type cell;tissue;serum;other liquid samples
Assay Length 30 min
Target Synonyms TOS
Reader Microplate reader
Test Principle Under acid conditions, the oxidizing material in the sample can oxidize Fe2+ to Fe3+, which binds highly with xylenol orange to produce a blue-purple complex. When the pH of solution is in the range of 2-3, its maximum absorption wavelength is around 590 nm, and the color depth is proportional to the content of oxidation substances in a certain concentration and a certain time, so as to indirectly calculate the total oxidation state of the sample.
Storage This product can be stored at 2~8°C/-20°C~-80°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
Technical Manual View Document
Msds View Document
Product Url View Document
Note The product is for research use only
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