Product Description
| Cat Number | E-BC-K611-M_96T |
|---|---|
| Category | Cell Metabolism Assay Kit |
| Pack Size | 96T |
| Detection Method | Colorimetric method |
| Research Field | Glycolysis and Lipid Metabolism;Tricarboxylic Acid（TCA）Cycle |
| Sensitivity | 0.08 U/L |
| Detection Range | 0.08–6.79 U/L |
| Sample Type | serum;plasma;tissue;cell |
| Assay Length | 10 min |
| Target Synonyms | PK |
| Reader | Microplate reader |
| Test Principle | In the presence of adenosine diphosphate (ADP), pyruvate kinase (PK) catalyzes the conversion of phosphoenolpyruvate (PEP) to pyruvate, Lactate dehydrogenase catalyzes the reaction of pyruvic acid with NADH to produce lactic acid and NAD+. The activity of pyruvate kinase can be calculated by detection the absorbance changes at 340 nm. |
| Storage | This product can be stored at 2~8°C/-20°C~-80°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Technical Manual | View Document |
| Msds | View Document |
| Product Url | View Document |
| Note | The product is for research use only |
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