Alanine Aminotransferase (ALT/GPT) Activity Assay Kit
SKU: BTL-EL-AK-02533 |
Brand: Elabscience
Product Description
| Cat Number | E-BC-K235-M_96T |
|---|---|
| Category | Cell Metabolism Assay Kit |
| Pack Size | 96T |
| Detection Method | Colorimetric method |
| Research Field | Liver and Renal Function |
| Sensitivity | 0.75 IU/L |
| Detection Range | 0.75-72.30 IU/L |
| Sample Type | serum;plasma;animal tissue;cells;cell culture supernatant |
| Assay Length | 80 min |
| Target Synonyms | ALT;GPT;Glutamic-pyruvic transaminase |
| Reader | Microplate reader |
| Test Principle | ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37℃. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm. |
| Storage | This product can be stored at 2~8°C/-20°C~-80°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| References | View Document |
| Technical Manual | View Document |
| Msds | View Document |
| Product Url | View Document |
| Note | The product is for research use only |
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