Low-Density Lipoprotein Cholesterol (LDL-C) Colorimetric Assay Kit (Double Reagents)
SKU: BTL-EL-AK-02503 |
Brand: Elabscience
Product Description
| Cat Number | E-BC-K205-S_100Assays |
|---|---|
| Category | Cell Metabolism Assay Kit |
| Pack Size | 100Assays |
| Detection Method | Colorimetric method |
| Research Field | Metabolic Diseases |
| Sensitivity | 0.061 mmol/L |
| Detection Range | 0.061–12 mmol/L |
| Sample Type | serum;plasma; animal tissue |
| Assay Length | 50 min |
| Reader | Spectrophotometer |
| Test Principle | Lipoproteins (except low-density lipoprotein cholesterol (LDL)) such as high density lipoprotein (HDL), chylomicron (CM), and very low density lipoprotein (VLDL) change structure and dissociate under the action of surfactants. The released micronized cholesterol molecules react with cholesterol enzyme reagents, and the generated hydrogen peroxide is trapped in the absence of coupling agent. It is consumed without color development. At this time, the LDL particles are still intact, and then the reagent containing coupling agent is added, which can dissociate the LDL particles to release cholesterol, which is catalyzed by cholesterol esterase (CE) and cholesterol oxidase (CO) and produce hydrogen peroxide. Hydrogen peroxide is catalyzed by oxidase (POD) in the presence of 4-aminoantipyrine (4-AA) and phenol (T-OOS) to form a red quinone compound, the shade of color is directly proportional to the LDL-C content. |
| Storage | This product can be stored at 2~8°C/-20°C~-80°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Technical Manual | View Document |
| Msds | View Document |
| Product Url | View Document |
| Note | The product is for research use only |
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