Homocysteine (Hcy) Colorimetric Assay Kit (Enzyme Circulation Method)
SKU: BTL-EL-AK-02427 |
Brand: Elabscience
Product Description
| Cat Number | E-BC-K143_100Assays |
|---|---|
| Category | Cell Metabolism Assay Kit |
| Pack Size | 100Assays |
| Detection Method | Colorimetric method |
| Research Field | Amino Acids and Proteins |
| Detection Range | 0-50 μmol/L |
| Sample Type | serum |
| Target Synonyms | Hcy |
| Reader | Biochemistry analyzer;Spectrophotometer |
| Test Principle | Oxidized homocysteine (HCY) is reduced to free homocysteine by triethyl phosphine (TCEP), and the free homocysteine reacts with substrate to generate adenosine. The generated adenosine is immediately dehydrogenated into inosine and ammonia, and the ammonia is further react with NADH under the catalysis of glutamate dehydrogenase to convert NADH to NAD+. The decrease in absorbance at 340 nm caused by the decline of NADH is proportional to the concentration of homocysteine in the sample. |
| Storage | This product can be stored at 2~8°C for 12 months with shading light. |
| Msds | View Document |
| Product Url | View Document |
| Note | The product is for research use only |
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