Reactive Oxygen Species (ROS) Fluorometric Assay Kit
SKU: BTL-EL-AK-02421 |
Brand: Elabscience
Product Description
| Cat Number | E-BC-K138-F_96T |
|---|---|
| Category | Cell Metabolism Assay Kit |
| Pack Size | 96T |
| Detection Method | Fluorometric method |
| Research Field | Oxidative Stress;Ferroptosis;Metabolic Diseases |
| Sample Type | Fresh tissue;cultured cells |
| Assay Length | 60 min |
| Target Synonyms | ROS;DCFH-DA;DCFH;DCF |
| Reader | Fluorescence Microplate reader;Fluorescence Microscope;Flow Cytometry |
| Test Principle | DCFH-DA (2,7-dichlorofuorescin diacetate) is a fluorescent probe without fluorescence that can freely cross the membrane. After entering the cell, it can be hydrolyzed by intracellular esterase to form DCFH (dichlorofluorescin). In the presence of reactive oxygen species (ROS), DCFH is oxidized to DCF (dichlorofluorescein) which is a strong green fluorescent substance that cannot penetrate the cell membrane. DCF has a maximum wave peak near the excitation wavelength of 502 nm and the emission wavelength of 525 nm, and the intensity is proportional to the level of intracellular reactive oxygen species. |
| Storage | This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| References | View Document |
| Technical Manual | View Document |
| Msds | View Document |
| Product Url | View Document |
| Note | The product is for research use only |
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