Product Description
| Cat Number | E-BC-K044-S_100Assays |
|---|---|
| Category | Cell Metabolism Assay Kit |
| Pack Size | 100Assays |
| Detection Method | Colorimetric method |
| Research Field | Glycolysis and Lipid Metabolism |
| Sensitivity | 0.05 mmol/L |
| Detection Range | 0.05-6.0 mmol/L |
| Sample Type | serum;plasma;cell culture supernatant;tissue;cells |
| Assay Length | 60 min |
| Reader | Spectrophotometer |
| Test Principle | Using NAD+ as hydrogen acceptor, LDH catalyzes the conversion of both lactate and NAD+ into pyruvic acid and NADH respectively. 1-Methoxy-5-methyl phenazine methyl sulfate (PMS) transfers hydrogen from NADH to NBT which deoxidize into purple chromogenic substrate. Lactic acid content can be calculated by measuring the OD value at 530 nm. |
| Storage | This product can be stored at 2~8°C for 12 months with shading light. |
| Technical Manual | View Document |
| Msds | View Document |
| Product Url | View Document |
| Note | The product is for research use only |
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