Non-esterified Free Fatty Acids (NEFA) Colorimetric Assay Kit
SKU: BTL-EL-AK-02225 |
Brand: Elabscience
Product Description
| Cat Number | E-BC-K014_96T |
|---|---|
| Category | Cell Metabolism Assay Kit |
| Pack Size | 96T |
| Detection Method | Colorimetric method |
| Research Field | Metabolic Diseases;Glycolysis and Lipid Metabolism |
| Detection Range | 0.01-3.0 mmol/L |
| Sample Type | serum;plasma;tissue homogenate;cells;cell supernatant |
| Target Synonyms | NEFA |
| Reader | Microplate reader;Biochemistry analyzer |
| Test Principle | NEFA and can react with coenzyme A and form acetyl-CoA under the catalysis of acetyl-CoA-synthetase (ACS). Acetyl-CoA can produce H2O2 when catalyzed by acetyl-CoA-oxidase (ACOD). Then H2O2 react with TOOS and 4-amino-antipyrine (4-APP) to generate a colored substrate under the catalysis of peroxidase (POD). The colored substrate has a maximum absorption peak at 546 nm. Measure the OD value at 546 nm and calculate the NEFA content indirectly. |
| Storage | This product can be stored at 2~8°C for 12 months with shading light. |
| Msds | View Document |
| Product Url | View Document |
| Note | The product is for research use only |
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