Inhibition and Production of Superoxide Anionic Colorimetric Assay Kit(WST-1 Method)
SKU: BTL-EL-AK-02195 |
Brand: Elabscience
Product Description
| Cat Number | E-BC-K001-M_500Assays |
|---|---|
| Category | Cell Metabolism Assay Kit |
| Pack Size | 500Assays |
| Detection Method | Colorimetric method |
| Research Field | Oxidative stress;Ferroptosis |
| Sample Type | serum;plasma;urine;cells;cell culture supernatant;leucocyte |
| Assay Length | 35 min |
| Reader | Microplate reader |
| Test Principle | Superoxide anion free radicals are produced through the reaction system of xanthine and xanthine oxidase. WST-1 (a water-soluble tetrazolium salt) can react with the generated superoxide anion to produce water-soluble formazan. When the tested sample contains the superoxide anion free radical inhibitor, it can inhibit the formation of formazan. When the tested sample contains the substance that produces superoxide anion free radical, it can promote the formation of formazan dye. By colorimetric analysis of WST-1 products, the units of activity of inhibition or production of superoxide anion radical in samples can be calculated. |
| Storage | This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| References | View Document |
| Technical Manual | View Document |
| Msds | View Document |
| Product Url | View Document |
| Note | The product is for research use only |
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