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Sphingomyelin (SM) Fluorometric Assay Kit

SKU: BTL-EL-AK-02177 | Brand: Elabscience
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Product Description

Cat Number E-BC-F079_96T
Category Cell Metabolism Assay Kit
Pack Size 96T
Detection Method Fluorescence method
Research Field Glycolysis and Lipid Metabolism
Sensitivity 0.35 μmol/L
Detection Range 0.35-40 μmol/L
Sample Type Serum;Plasma;Animal tissue;Cell
Assay Length 1 h 30 min
Reader Fluorescence microplate reader
Test Principle Sphingomyelin (SM) is widely present in cell membranes and plasma lipoproteins. Metabolism of SM is regulated by sphingomyelin synthase and sphingomyelase, whose metabolites include ceramide, sphingosinol and choline phosphate, which are important second messengers. Studies have shown that SM and its metabolites play an important role in liver, lung, and cardiovascular diseases. The principle of this kit is that SM is catalyzed by sphingomyelin enzyme, and its products generate fluorescent substances with the probe under the action of other enzymes. By substituting the fluorescence value into the standard curve, SM content in the sample can be calculated.
Storage This product can be stored at -20°C for 12 months with shading light.
Technical Manual View Document
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Note The product is for research use only
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