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Acetyl Coenzyme A (Acetyl-CoA) Fluorometric Assay Kit

SKU: BTL-EL-AK-02139 | Brand: Elabscience
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Product Description

Cat Number E-BC-F046_96T
Category Cell Metabolism Assay Kit
Pack Size 96T
Detection Method Fluorescence method
Research Field Tricarboxylic Acid(TCA)Cycle
Sensitivity 0.27 μmol/L
Detection Range 0.27-5.00 μmol/L
Sample Type Serum;Plasma;Animal tissue;Cell
Assay Length 30 min
Reader Fluorescence microplate reader
Test Principle Acetyl-CoA is an essential cofactor and acyl carrier in the enzymatic acetyl transfer reaction. It can be formed by oxidative decarboxylation of pyruvate in mitochondria, oxidation of long-chain fatty acids, or oxidative degradation of certain amino acids. Acetyl-CoA is the starting compound of the citric acid cycle.
The detection principle of this kit is that Acetyl-CoA is converted into other substances under the condition of enzyme catalysis, and the resulting fluorescence is detected by fluorescence microplate reader at the maximum excitation wavelength of 535 nm and the maximum emission wavelength of 587 nm.
Storage This product can be stored at -20°C for 12 months with shading light.
Technical Manual View Document
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Note The product is for research use only
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