Product Description
| Cat Number | E-BC-F042_96T |
|---|---|
| Category | Cell Metabolism Assay Kit |
| Pack Size | 96T |
| Detection Method | Fluorometric method |
| Research Field | Glycolysis and Lipid Metabolism;Plant Physiology Series |
| Sensitivity | 0.15 μmol/L |
| Detection Range | 0.15-15 μmol/L |
| Sample Type | plant tissue |
| Assay Length | 70 min |
| Reader | Fluorescence microplate reader |
| Test Principle | Sucrose can be hydrolyzed by sucrase to produce glucose under acidic conditions, which is catalyzed by glucose oxidase to produce hydrogen peroxide. In the presence of HRP (horse radish peroxidase), hydrogen peroxide reacts with the fluorescent probe to form red fluorescent substance. The sucrose content can be calculated by measuring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 590 nm. |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Technical Manual | View Document |
| Msds | View Document |
| Product Url | View Document |
| Note | The product is for research use only |
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