Free Fatty Acids (NEFA/FFA) Fluorometric Assay Kit
SKU: BTL-EL-AK-02128 |
Brand: Elabscience
Product Description
| Cat Number | E-BC-F039_48T |
|---|---|
| Category | Cell Metabolism Assay Kit |
| Pack Size | 48T |
| Detection Method | Fluorometric method |
| Research Field | Metabolic Diseases;Glycolysis and Lipid Metabolism |
| Sensitivity | 1.20 μmol/L |
| Detection Range | 1.20-100 μmol/L |
| Sample Type | serum;plasma;animal tissue;cells |
| Assay Length | 70 min |
| Target Synonyms | FFA;NEFA |
| Reader | Fluorescence Microplate Reader |
| Test Principle | Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen peroxide in the presence of acyl oxidase. In the presence of the enzyme and probe, hydrogen peroxide react to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is directly proportional to the concentration of free fatty acids. |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Technical Manual | View Document |
| Msds | View Document |
| Product Url | View Document |
| Note | The product is for research use only |
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