Alanine Aminotransferase (ALT/GPT) Activity Fluorometric Assay Kit
SKU: BTL-EL-AK-02126 |
Brand: Elabscience
Product Description
| Cat Number | E-BC-F038_48T |
|---|---|
| Category | Cell Metabolism Assay Kit |
| Pack Size | 48T |
| Detection Method | Fluorometric method |
| Research Field | Liver and Renal Function |
| Sensitivity | 0.01 U/L |
| Detection Range | 0.01-0.83 U/L |
| Sample Type | serum;plasma;animal tissue |
| Assay Length | 75 min |
| Target Synonyms | ALT |
| Reader | Fluorescence Microplate Reader |
| Test Principle | ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid. Under the action of pyruvate oxidase, pyruvic acid generates H2O2, which reacts with the non-fluorescent substance to form fluorescent substance under the action of peroxidase. The activity of ALT can be calculated by measuring the increase of fluorescence value at the excitation wavelength of 535 nm and the emission wavelength of 590 nm. |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| References | View Document |
| Technical Manual | View Document |
| Msds | View Document |
| Product Url | View Document |
| Note | The product is for research use only |
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