Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit
SKU: BTL-EL-AK-02118 |
Brand: Elabscience
Product Description
| Cat Number | E-BC-F032_48T |
|---|---|
| Category | Cell Metabolism Assay Kit |
| Pack Size | 48T |
| Detection Method | Fluorometric method |
| Research Field | Glycolysis and Lipid Metabolism |
| Sensitivity | 0.12 μmol/L |
| Detection Range | 0.12-30 μmol/L |
| Sample Type | serum;plasma;animal tissue;cells |
| Assay Length | 20 min |
| Reader | Fluorescence Microplate Reader |
| Test Principle | Total Cholesterol (TC) includes free cholesterol (FC) and cholesteryl esters (CE). Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce △4-cholestenone and hydrogen peroxide. In the presence of the enzyme and probe, hydrogen peroxide can be catalyzed to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is proportional to the cholesterol concentration. |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| References | View Document |
| Technical Manual | View Document |
| Msds | View Document |
| Product Url | View Document |
| Note | The product is for research use only |
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