Ferroptosis Inhibitory Protein-1 (FSP-1) Activity Fluorometric Assay Kit
SKU: BTL-EL-AK-02115 |
Brand: Elabscience
Product Description
| Cat Number | E-BC-F022_96T |
|---|---|
| Category | Cell Metabolism Assay Kit |
| Pack Size | 96T |
| Detection Method | Fluorescence method |
| Research Field | Ferroptosis |
| Sensitivity | 0.15 U/L |
| Detection Range | 0.15-5.00 U/L |
| Sample Type | Animal and plant tissue;Cell |
| Assay Length | 1 h |
| Reader | Fluorescence microplate reader |
| Test Principle | Ferroptosis inhibitory protein-1(FSP-1) induce non-caspase-dependent apoptosis based on factors such as C-terminal fragment of amino acid series, nuclear translocation and overexpression. Through the FSP1-CoQ10-NAD(P)H pathway and the classical glutathione (GSH)-GPX4 pathway, FSP-1 can protect cells from ferroptosis, and plays a "double-edged sword" role in cell life activities. The detection principle of this kit: FSP-1 catalyzes the substrate reaction to generate NADH, so that its fluorescence value increases under the excitation wavelength of 535 nm and emission wavelength of 587 nm. Adding inhibitors will inhibit the activity of FSP-1 and cause the rate of increase in fluorescence value to decrease, and the activity of FSP-1 can be calculated by measuring the difference value. |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Technical Manual | View Document |
| Msds | View Document |
| Product Url | View Document |
| Note | The product is for research use only |
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