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ATP/ADP Ratio Chemiluminescence Assay kit

SKU: BTL-EL-AK-02099 | Brand: Elabscience
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Product Description

Cat Number E-BC-F004_48T
Category Cell Metabolism Assay Kit
Pack Size 48T
Detection Method Fluorescence method
Research Field Glycolysis and Lipid Metabolism
Sample Type cell
Assay Length 60 min
Reader Chemiluminescence immunoassay analyzer or multifunctional microplate reader(with chemiluminescence)
Test Principle Adenosine triphosphate (ATP) is the energy currency of organisms, produced by glycolysis or the tricarboxylic acid cycle and electron transport chain in mitochondria, and is the source of energy in cells. Under normal circumstances, ATP and adenosine diphosphate (ADP) will convert to each other, and the change of the ratio of ATP to ADP is closely related to apoptosis, autophagy, energy metabolism and other pathways, so it is often used as an indicator of cell activity. Under the catalysis of luciferase, ATP react with luciferin and emits fluorescence. Convert ADP to ATP by enzyme, and ATP was detected by the same principle. The ATP/ADP ratio in the cell can be calculated.
Storage This product can be stored at -20°C for 12 months with shading light.
Technical Manual View Document
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Note The product is for research use only
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