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Lipid Peroxide (LPO) Fluorometric Assay Kit

SKU: BTL-EL-AK-02098 | Brand: Elabscience
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Product Description

Cat Number E-BC-F003_96T
Category Cell Metabolism Assay Kit
Pack Size 96T
Detection Method Fluorometric method
Research Field Ferroptosis
Sample Type cell
Target Synonyms LPO
Reader Fluorescence Microplate reader
Test Principle Lipid peroxides (LPO) are substances produced by the action of reactive oxygen species (ROS) on unsaturated fatty acids. The LPO produced by the reaction will continue to oxidize the liposomes, thereby accumulating LPO in a chain reaction. Excess LPO decomposes into highly active aldehydes such as Acrolein, Malondialdehyde (MDA), and 4-hydroxy-2-nonenal (4-NHE), causing cell toxicity and cell death. This kit provides a fluorescent probe that can detect LPO production. This fluorescent probe can react with the lipid free radicals in the lipid peroxidation pathway, so as to detect the level of lipid peroxidation in cells. The probe fluoresces red under normal conditions through photoexcitation, and the fluorescence changes from red to green with the process of lipid peroxidation. Through the enhancement of green fluorescence intensity, the production of LPO can be detected with high sensitivity.
Storage This product can be stored at -20°C for 12 months with shading light.
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Note The product is for research use only
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