Human CD8 alpha&beta Heterodimer Protein, Ultra Low Endotoxin (CD8-HM1AB-UL)
SKU: BTL-KB-P-00805 |
Host: Human |
Brand: Kactus Bio
Product Description
| Cat Number | CD8-HM1AB-UL |
|---|---|
| Category | Recombinant Protein |
| Pack Size | 100UG |
| Description | Recombinant Human CD8 alpha&beta Heterodimer Protein is expressed from HEK293 with His tag at the C-Terminus of CD8 alpha and Flag tag at the C-Terminus of CD8 beta.It contains Ser22-Asp182(CD8 alpha) acidic tail and Leu22-Pro170(CD8 beta) basic tail. |
| Target | CD8 alpha&beta |
| Host | Human |
| Purity | > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
| Scientific Background | CD8 alpha&beta (CD8αβ) is a heterodimeric form of CD8. CD8α is required for surface expression of CD8β. The extracellular IgV-like domain of CD8α interacts with the α3 portion of the class I MHC molecule. CD8αβ is expressed on human peripheral T cells and functions as a coreceptor and can greatly increase the sensitivity and breadth of antigen recognition by CD8+ peripheral T cells bearing TCR. |
| Molecular Mass | The protein has a predicted MW of 23.54 kDa (CD8 alpha) and 22.62 kDa (CD8 beta). Due to glycosylation, the protein migrates to 30-45 kDa based on Bis-Tris PAGE result. |
| Sl Sequence | Ser22-Asp182(CD8 alpha) acidic tail & Leu22-Pro170(CD8 beta) basic tail |
| Solubility | Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
| Appearance | Lyophilized |
| Endotoxin Levels | Less than 0.01 EU per μg by the LAL method. |
| Expression System | HEK293 |
| Gene Id | P01732-1(CD8 alpha)&P10966-1(CD8 beta) |
| Amino Acid Sequence | Recombinant Human CD8 alpha&beta Heterodimer Protein is expressed from HEK293 with His tag at the C-Terminus of CD8 alpha and Flag tag at the C-Terminus of CD8 beta.It contains Ser22-Asp182(CD8 alpha) acidic tail and Leu22-Pro170(CD8 beta) basic tail. |
| Storage | -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| View Document | |
| Note | The product is for research use only |
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