TransScript® Uni Reverse Transcriptase[M-MLV, RNase H-](High Temperature RT)
SKU: BTL-TB-MB-00152 |
Brand: Transgen Biotek
Product Description
| Cat Number | AU101-02 |
|---|---|
| Category | Molecular Biology Product |
| Pack Size | 10000 units |
| Description | TransScript® Uni Reverse Transcriptase is a reverse transcriptase with high thermal stability expressed and purified from E. coli after genetic modification and screening. It enables a broard range of reaction temperature (42°C-65°C) with ultra-high thermal stability and deficient RNase H activity. Its high reaction temperature is beneficial to open RNA secondary structure when synthesizing the first-strand cDNA, with the optimal reaction temperature of 50°C. It also has the characteristics of high sensitivity, high specificity, high yield (more full-length cDNA), High thermal stabilityand long half-life. Features · Ultra-high thermal stability: reaction temperature 42°C-65°C. · High sensitivity, high specificity, high highyield and high processivity. · The unique design of Anchored Oligo (dT)20 can anchor the first base next to the mRNA Poly (A)+5’end, and the binding site is anchored, with high specificity, ensuring the efficiency and success rate of first-strand cDNA synthesis. · Deficient RNase H activity. The degradation of the template RNA in the DNA/RNA hybrid in the first-strand cDNA synthesis reaction is avoided, thereby ensuring the yieldand length of the first-strand cDNA. · Synthesis length can be up to 20kb. Applications · High-copy number and low-copy number gene detection. · High GC or RNA template with complex secondary structure. · cDNA library construction, primer extension, 3’ and 5’ RACE. Storage at -20 ℃ for two years Shipping Dry ice (-70 ℃) |
| Storage | -80°C |
| Note | The product is for research use only |
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