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Creatinine (Cr) Colorimetric Assay Kit (Sarcosine Oxidase Method)

SKU: BTL-EL-AK-02490 | Brand: Elabscience
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Product Description

Cat Number E-BC-K188-M_96T
Category Cell Metabolism Assay Kit
Pack Size 96T
Detection Method Colorimetric method
Research Field Liver and Renal Function
Sensitivity 3.8 μmol/L
Detection Range 20.45-400 μmol/L
Sample Type serum;plasma;urine
Assay Length 30 min
Target Synonyms Cr
Reader Microplate reader
Test Principle Creatinine (Cr) can be catalyzed by creatinase and generates creatine. Creatine can be hydrolyzed into sarcosine and urea by creatinase. The sarcosine can be catalyzed by sarcosine oxidase and form glycine, formaldehyde and hydrogen peroxide. The reaction between hydrogen peroxide, 2,4-(6-Tri-iodine-3- hydroxybenzoic acid) and 4-ampyrone can be catalyzed by peroxidase and form pink compound. Creatinine content can be calculated indirectly by measuring the OD value at 515 nm.
Storage This product can be stored at 2~8°C/-20°C~-80°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
References View Document
Technical Manual View Document
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Note The product is for research use only
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