Total Bile Acid (TBA) Colorimetric Assay Kit
SKU: BTL-EL-AK-02483 |
Brand: Elabscience
Product Description
| Cat Number | E-BC-K181-M_96T |
|---|---|
| Category | Cell Metabolism Assay Kit |
| Pack Size | 96T |
| Detection Method | Colorimetric method |
| Research Field | Liver and Renal Function |
| Sensitivity | 1.36 μmol/L |
| Detection Range | 1.77-40 μmol/L |
| Sample Type | serum;animal tissue |
| Assay Length | 25 min |
| Target Synonyms | TBA |
| Reader | Microplate reader |
| Test Principle | With S-NAD+ as hydrogen receptor, 3α-hydroxy steroid dehydrogenase catalyzed the dehydrogenation of bile acids to produce 3-ketone steroids, transforming S-NAD+ into S-NADH. Meanwhile, NADH was used as hydrogen donor. 3α-hydroxy steroid dehydrogenase catalyzed the production of bile acids from 3-ketone steroids. Through the enzyme cycle reaction, S-NADH is continuously generated, which has the maximum absorption peak at 405 nm. Measure the OD value at 405 nm and the changes of absorbance is proportional to the concentration of bile acid. |
| Storage | This product can be stored at 2~8°C/-20°C~-80°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| References | View Document |
| Technical Manual | View Document |
| Msds | View Document |
| Product Url | View Document |
| Note | The product is for research use only |
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