TransScript® Uni All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal)
SKU: BTL-TB-MB-00153 |
Brand: Transgen Biotek
Product Description
| Cat Number | AU341-02 |
|---|---|
| Category | Molecular Biology Product |
| Pack Size | 100 rxns |
| Description | The kit provides all the necessary reagents for reverse transcription reaction (including TransScript® Uni RT, RNase Inhibitor, Anchored Oligo (dT)20 Primer, Random Primer (N9), dNTPs, Buffer). It is provided at 5× concentration and used by only adding gDNA remover, RNA template and H2O at 42°C-65°C for efficient first-strand cDNA synthesis. Simultaneously, residual genomic DNA from RNA template can be removed. 5×TransScript® Uni All-in-One No-RT Control SuperMix for qPCR is supplied to prepare no-reverse transcriptase (RT) control, which is used to assess if the qPCR template is contaminated with genomic DNA. This product is capable of minimizing contamination during operation with a simple workflow. The resulting cDNA is only suitable for qPCR, not for regular PCR. Highlights • Board range reaction temperature (42°C-65°C). • "All-in-One SuperMix" form: Simultaneous cDNA synthesis and genomic DNA removal by only adding gDNA remover, RNA template and H2O • High synthesis efficiency enabled by optimal ratio of Oligo(dT)20 Primer to Random Primer (N9) and optimized composition of the SuperMix, ensuring same reverse transcription efficiency for RNA templates of different concentrations and specifically high synthesis efficiency for short cDNA. • Only 5 minutes for reverse transcription. • High compatibility with qPCR reagents. Applications High-copy number and low-copy number gene detection Storage at -20 ℃ for two years Shipping Dry ice (-70 ℃) |
| Storage | -80°C |
| References | 1 Qin T, Zhang G, Zheng Y, et al. A population of stem cells with strong regenerative potential discovered in deer antlers[J]. Science, 2023.(IF 63.71) 2 Yang L, Wu X, Liu S, et al. Comprehensive Analysis of BrHMPs Reveals Potential Roles in Abiotic Stress Tolerance and Pollen–Stigma Interaction in Brassica rapa[J]. Cells, 2023.(IF 7.66) 3 Yang X, Liu S, Lu W, et al. Delta and jagged are candidate target genes of RNAi biopesticides for the control of Nilaparvata lugens[J]. Frontiers in Bioengineering and Biotechnology, 2022.(IF 6.064) 4 Yuan Y, Fu D, Xu Y, et al. Pt (IV) Prodrug as a Potential Antitumor Agent with APE1 Inhibitory Activity[J]. Journal of Medicinal Chemistry, 2022.(IF 8.039) |
| Note | The product is for research use only |
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