TransScript® Uni One-Step gDNA Removal and cDNA Synthesis SuperMix
SKU: BTL-TB-MB-00133 |
Brand: Transgen Biotek
Product Description
| Cat Number | AU311-02 |
|---|---|
| Category | Molecular Biology Product |
| Pack Size | 50 rxns×20 µl system |
| Description | TransScript®-Uni RT is an improved version of M-MLV reverse transcriptase with broad range of reaction temperature(42°C-65°C) and higher thermostability. The suggested reaction temperature is 50°C. The SuperMix contains reagents for simultaneous genomic DNA removal and cDNA synthesis. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85°C for 5 seconds. • Broad range reaction temperature (42°C -65°C ) . • Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination. • The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR. • cDNA up to 20 kb. Applications • Multiple copy and low copy gene detection • GC-rich or complex secondary structure RNA template • cDNA library construction, primer extension, 3’ and 5’ RACE Storage at -20 ℃ for two years Shipping Dry ice (-70 ℃) |
| Storage | -80°C |
| References | 1 Li C, Guan H, Jing X, et al. Genomic insights into historical improvement of heterotic groups during modern hybrid maize breeding[J]. Nature Plants, 2022.(IF 17.35) 2 Chen L, Zhang C, Ma W, et al. METTL3-mediated m6A modification stabilizes TERRA and maintains telomere stability[J]. Nucleic Acids Research, 2022.(IF 19.16) 3 Wang Y, Gong Q, Wu Y, et al. A calmodulin-binding transcription factor links calcium signaling to antiviral RNAi defense in plants[J]. Cell Host & Microbe, 2021.(IF 21.02) 4 Kumar S, Wang G, Liu W, et al. Hypoxia-induced mitogenic factor promotes cardiac hypertrophy via calcium-dependent and hypoxia-inducible factor-1α mechanisms[J]. Hypertension, 2018.(IF 6.82) |
| Note | The product is for research use only |
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