TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal )<
SKU: BTL-TB-MB-00142 |
Brand: Transgen Biotek
Product Description
| Cat Number | AT341-01 |
|---|---|
| Category | Molecular Biology Product |
| Pack Size | 50 rxns×20 µl system |
| Description | The kit provides all the necessary components for cDNA synthesis from total RNA or mRNA. It is provided at 5× concentration and used at 1× concentration by adding gDNA remover, RNA and H2O. Simultaneous genomic DNA removal and cDNA synthesis are performed. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85℃ for 5 seconds. The resulting cDNA is suitable for qPCR, not for regular PCR. • Simultaneous genomic DNA removal and cDNA synthesis. • The optimal ratio of oligo(dT)18 primer to random primer(N9) for qPCR ready cDNA. • qPCR ready cDNA in 15 minutes. • cDNA up to 250 bp. Applications Multiple copy and low copy gene detection Storage at -20 ℃ for two years Shipping Dry ice (-70 ℃) |
| Storage | -80°C |
| References | 1 Sun X, Zhang T, Tong B, et al. POGZ suppresses 2C transcriptional program and retrotransposable elements[J]. Cell Reports, 2023.(IF 8.80) 2 Yuan F, Cai J, Wu J, et al. Z-DNA binding protein 1 promotes heatstroke-induced cell death[J]. Science, 2022.(IF 47.72) 3 Yuan F, Cai J, Wu J, et al. Z-DNA binding protein 1 promotes heatstroke-induced cell death[J]. Science, 2022.(IF 63.71) 4 Yu Z, Deng P, Chen Y, et al. Inhibition of the PLK1‐coupled cell cycle machinery overcomes resistance to oxaliplatin in colorectal cancer[J]. Advanced Science, 2021.(IF 16.80) 5 Sun X, Peng X, Cao Y, et al. ADNP promotes neural differentiation by modulating Wnt/β-catenin signaling[J]. Nature communications, 2020.(IF 12.12) 6 Wu H, Qu X, Dong Z, et al. WUSCHEL triggers innate antiviral immunity in plant stem cells[J]. Science, 2020.(IF 41.84) 7 Ji S, Luo Y, Cai Q, et al. LC domain-mediated coalescence is essential for Otu enzymatic activity to extend Drosophila lifespan[J]. Molecular cell, 2019.(IF 14.20) |
| Note | The product is for research use only |
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