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TransStart® FastPfu DNA Polymerase (with 2.5 mM dNTPs)

SKU: BTL-TB-MB-00056 | Brand: Transgen Biotek
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Product Description

Cat Number AP221-11
Category Molecular Biology Product
Pack Size 250 units
Description TransStart ® FastPfu DNA Polymerase is a fast, high fidelity and high processivity hot start DNA polymerase. • Extension rate is about 2-4 kb/min. • TransStart ® FastPfu DNA Polymerase offers 54-fold fidelity as compared to EasyTaq® DNA Polymerase. • PCR products can be directly cloned into pEASY®-Blunt vectors. • Amplification of genomic DNA fragment up to 15 kb. • Amplification of plasmid DNA fragment up to 20 kb. Applications • High fidelity PCR • High yield and fast PCR • Blunt end cloning • Site-directed mutagenesis • Complex templates Storage at -20 ℃ for two years Shipping Dry ice (-70 ℃)
Storage -80°C
References 1 Fan J, Ran H, Wei P L, et al. Pretrichodermamide A Biosynthesis Reveals the Hidden Diversity of Epidithiodiketopiperazines[J]. Angewandte Chemie, 2023.(IF 16.82) 2 Wang Y, Zhang S, Yang X, et al. Mesoscale DNA feature in antibody-coding sequence facilitates somatic hypermutation[J]. Cell, 2023.(IF 66.00) 3 Fan J, Ran H, Wei P L, et al. An ortho‐Quinone Methide Mediates Disulfide Migration in the Biosynthesis of Epidithiodiketopiperazines[J]. Angewandte Chemie International Edition, 2023.(IF 16.82) 4 Zong Y, Liu Y, Xue C, et al. An engineered prime editor with enhanced editing efficiency in plants[J]. Nature Biotechnology, 2022.(IF 54) 5 Lei Y, Fei P, Song B, et al. A loosened gating mechanism of RIG-I leads to autoimmune disorders[J]. Nucleic acids research, 2022.(IF 16.971) 6 Zhang H, Zhu Y, Liu Z, et al. A volatile from the skin microbiota of flavivirus-infected hosts promotes mosquito attractiveness[J]. Cell, 2022.(IF 66.85) 7 Zhang H, Li Z, Zhou S, et al. A fungal NRPS-PKS enzyme catalyses the formation of the flavonoid naringenin[J]. Nature Communications, 2022.(IF 17.694) 8 Li Y, Zhao L, Zhang Y, et al. Structural basis for product specificities of MLL family methyltransferases[J]. Molecular Cell, 2022.(IF 19.328) 9 Lei Z, Meng H, Liu L, et al. Mitochondrial base editor induces substantial nuclear off-target mutations[J]. Nature, 2022.(IF 69.50)   10 Lin Q, Jin S, Zong Y, et al. High-efficiency prime editing with optimized, paired pegRNAs in plants[J]. Nature Biotechnology, 2021.(IF 54.90) 11 Song B, Chen Y, Liu X, et al. Ordered assembly of the cytosolic RNA-sensing MDA5-MAVS signaling complex via binding to unanchored K63-linked poly-ubiquitin chains[J]. Immunity, 2021.(IF 31.74) 12 Zong Y, Liu Y, Xue C, et al. An engineered prime editor with enhanced editing efficiency in plants[J]. Nature Biotechnology, 2022.(IF 54.00) 13 Li C, Zhang R, Meng X, et al. Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors[J]. Nature biotechnology, 2020.(IF 35.72) 14 Lin Q, Zong Y, Xue C, et al. Prime genome editing in rice and wheat[J]. Nature biotechnology, 2020.(IF 31.90) 15 Wang S, Zong Y, Lin Q, et al. Precise, predictable multi-nucleotide deletions in rice and wheat using APOBEC–Cas9[J]. Nature biotechnology, 2020.(IF 36.55) 16 Chen J, Ou Y, Yang Y, et al. KLHL22 activates amino-acid-dependent mTORC1 signalling to promote tumorigenesis and ageing[J]. Nature, 2018.(IF 40.13)
Note The product is for research use only
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