Product Description
| Cat Number | AP151-02 |
|---|---|
| Category | Molecular Biology Product |
| Pack Size | 500 units |
| Description | TransStart® TopTaq DNA Polymerase is an engineered version of Taq DNA Polymerase combined with TransStart® technique. One binding protein binds to double-strand DNA template, preventing polymerase activity at room temperature. Other two binding proteins bind primers, preventing primer-dimer formation. Blocking proteins are released from primers and templates during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR. • Compared with TransStart® Taq DNA Polymerase, TransStart®TopTaq DNA Polymerase has higher amplification efficiency, specificity and sensitivity. • TransStart® TopTaq DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase. • The specificity is higher than antibody based or chemically modified hot start DNA polymerases. • Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors. • Reduced nonspecific amplification and primer dimer formation. • Different from Taq antibody, no risk of contamination from mammalian DNA. • Different from chemical modification, long denaturing step is not needed. • Amplification of genomic DNA fragment up to 15 kb. Applications • Complex templates • GC/AT-rich templates • Multiplex PCR • High yield PCR Storage at -20 ℃ for two years Shipping Dry ice (-70 ℃) |
| Storage | -80°C |
| References | Su Y , Li B , Zhu W Y . Fecal microbiota of piglets prefer utilizing dl-lactate mixture as compared to d-lactate and l-lactate in vitro[J]. Anaerobe, 2013, 19(Complete):27-33. |
| Note | The product is for research use only |
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