TransScript® II One-Step gDNA Removal and cDNA Synthesis SuperMix
SKU: BTL-TB-MB-00140 |
Brand: Transgen Biotek
Product Description
| Cat Number | AH311-03 |
|---|---|
| Category | Molecular Biology Product |
| Pack Size | 100 rxns×20 µl system |
| Description | Unique genomic DNA remover is combined with TransScript® II First- Strand cDNA Synthesis SuperMix to achieve simultaneous genomic DNA removal and cDNA synthesis at 42-55°C. After cDNA synthesis, gDNA remover and TransScript® II RT/RI Enzyme Mix are inactivated by heating at 85°C for 5 seconds. • Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination. • The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR. • After reaction, heat inactivation for RT/RI and gDNA remover simultaneously can avoid damage to RNA caused by heat inactivating DNase I used for pretreatment in traditional protocol. • Simple and fast procedure. • cDNA up to 15 kb. Applications • cDNA library construction, 3’ and 5’ RACE • Multiple copy and low copy gene detection • GC-rich or complex secondary structure RNA template Storage at -20 ℃ for two years Shipping Dry ice (-70 ℃) |
| Storage | -80°C |
| References | 1 Geng Q, Li H, Wang D, et al. The Verticillium dahliae Spt-Ada-Gcn5 acetyltransferase complex subunit Ada1 is essential for conidia and microsclerotia production and contributes to virulence[J]. Frontiers in Microbiology, 2022.(IF 6.06) 2 Zhang Y, Fan Y, Rui C, et al. Melatonin improves cotton salt tolerance by regulating ROS scavenging system and Ca2+ signal transduction[J]. Frontiers in Plant Science, 2021.(IF 5.75) 3 Cui B, Luo Y, Tian P, et al. Stress-induced epinephrine enhances lactate dehydrogenase A and promotes breast cancer stem-like cells[J]. The Journal of clinical investigation, 2019.(IF 13.25) 4 Zhao J, Liu Y, Lin F, et al. Bioorthogonal Engineering of Bacterial Effectors for Spatial–Temporal Modulation of Cell Signaling[J]. ACS Central Science, 2018.(IF 11.22) 5 Liu G, Yan P, Du Q, et al. Pre-rRNA processing and its response to temperature stress in maize[J]. Journal of Experimental Botany, 2020.(IF 5.36) 6 He L, Liu Y, He H, et al. A molecular framework underlying the compound leaf pattern of Medicago truncatula[J]. Nature Plants, 2020.(IF 13.25) |
| Note | The product is for research use only |
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