TransScript® II First-Strand cDNA Synthesis SuperMix
SKU: BTL-TB-MB-00138 |
Brand: Transgen Biotek
Product Description
| Cat Number | AH301-03 |
|---|---|
| Category | Molecular Biology Product |
| Pack Size | 100 rxns×20 µl system |
| Description | TransScript® II First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA. The cDNA is efficiently synthesized by TransScript® II RT/RI Enzyme Mix and 2×TS II Reaction Mix at 42-55℃, of which the optimum temperature is 50℃. • Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis to ensure its synthesis length and yield. • The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR. • Anchored Oligo(dT)20 Primer is specifically designed to bind to the first base next to the 5' end of Poly(A) tail of mRNA, providing higher specificity and high efficiency for first-strand cDNA synthesis. • cDNA up to 15 kb. Applications • cDNA library construction, 3’ and 5’ RACE • Multiple copy and low copy gene detection • GC-rich or complex secondary structure RNA template Storage at -20 ℃ for two years Shipping Dry ice (-70 ℃) |
| Storage | -80°C |
| References | 1 Zhao X, Lian X, Xie J, et al. Accumulated cholesterol protects tumours from elevated lipid peroxidation in the microenvironment[J]. Redox Biology, 2023.(IF 10.78) |
| Note | The product is for research use only |
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