EasyScript® All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal)&
SKU: BTL-TB-MB-00124 |
Brand: Transgen Biotek
Product Description
| Cat Number | AE341-02 |
|---|---|
| Category | Molecular Biology Product |
| Pack Size | 100 rxns×20 µl system |
| Description | 5×EasyScript® All-in-One SuperMix for qPCR provides all the necessary reagents for cDNA synthesis from total RNA or mRNA (including 5×EasyScript® RT, RNase Inhibitor, Anchored Oligo (dT)18 Primer, Random Primer (N9), dNTPs, Buffer). It is provided at 5× concentration and used at 1× concentration by only adding gDNA remover, RNA template and H2O for efficient first-strand cDNA synthesis. Simultaneously, residual genomic DNA from RNA template can be removed. 5×EasyScript® All-in-One No-RT Control SuperMix for qPCR is supplied to prepare no-reverse transcriptase (RT) control, which is used to assess if the qPCR template is contaminated with genomic DNA. This product is capable of minimizing contamination during operation with a simple workflow. The resulting cDNA is only suitable for qPCR, not for regular PCR. Highlights • “All-in-One SuperMix” type: Simultaneous cDNA synthesis and genomic DNA removal by only adding gDNA remover, RNA template and H2O • High synthesis efficiency enabled by optimal ratio of Oligo(dT)18 Primer to Random Primer (N9) and optimized composition of the SuperMix, ensuring same reverse transcription efficiency for RNA templates of different concentrations and specifically high synthesis efficiency for short cDNA. • Only 15 minutes for reverse transcription. • High compatibility with qPCR reagents. Applications High-copy-number and low-copy number gene detection Storage at -20 ℃ for two years Shipping Dry ice (-70 ℃) |
| Storage | -80°C |
| References | 1 He F, Cheng K, Qi J, et al. Black phosphorus nanosheets enhance differentiation of neural progenitor cells for improved treatment in spinal cord injury[J]. Chemical Engineering Journal, 2023.(IF 15.10) 2 Liu X, Hou S, Xiang R, et al. Imipramine activates FAM3A-FOXA2-CPT2 pathway to ameliorate hepatic steatosis[J]. Metabolism, 2022.(IF 13.93) 3 He F, Liu Z, Xu J, et al. Black phosphorus nanosheets suppress oxidative damage of stem cells for improved neurological recovery[J]. Chemical Engineering Journal, 2023.(IF 16.74) 4 Yin Q, Li Y, Zhou Z, et al. RPA1 controls chromatin architecture and maintains lipid metabolic homeostasis[J]. Cell Reports, 2022.(IF 9.99) 5 Zhang Y, Zhang M, Yao A, et al. Circ_0011129 Encapsulated by the Small Extracellular Vesicles Derived from Human Stem Cells Ameliorate Skin Photoaging[J]. International Journal of Molecular Sciences, 2022.(IF 6.20) |
| Note | The product is for research use only |
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