Multiplex ELISA kits - by Quansys Biosciences


Multiplex ELISA kits - by Quansys Biosciences



Since few decades enormous research had been going on stating the role of inflammation in aging, disability, age-related diseases, and frailty.  Hence the availability of methods for measuring cytokines and other inflammatory markers with high specificity and sensitivity is crucially important. As we all know Enzyme-Linked Immuno-Sorbant Assay (ELISA) is the most widely used and best-validated method for this. It is considered as the standard cytokine measurement method and is used in biomedical research on a large scale. ELISA kits for various cytokines are available with different companies and vendors. It has its own advantages that include results are highly quantitative and generally reproducible. Along with these ELISA has its own limitation like its performance is mainly dependent on the antibody, kit manufacturer, and experience. It also allows the measurement of one cytokine at a time in a given sample. This shortcoming limits the ability of ELISA to meet researchers' need to include ever-growing numbers of inflammatory molecules in their research. This concern becomes more acute when such studies require access to limited amounts of biological samples obtained from older adults in the course of longitudinal studies. Another limitation of ELISA-based assays is that the dynamic range (range over which there is a linear relationship between the concentration of cytokine and the absorbance reading) is narrow relative to the range for other technologies such as multiplex assays.

This limitation has led to the development of multiplex arrays. These have been developed from traditional ELISA with an interest to measure multiple cytokines at the same time in a same sample. They are available in several different formats based on the utilization of flow cytometry, chemiluminescence, or electrochemiluminescence technology. Flow cytometric multiplex arrays, also known as bead-based multiplex assays, represent probably the most commonly used format at the present time. Multiplex Arrays offer the potential of better evaluating the complexity and dynamic nature of inflammatory responses and offer substantial cost and sample savings over traditional ELISA measurements.

Multiplex arrays have numerous advantages including:

a) high throughput multiplex analysis

b) low volume of sample needed

c) both time and cost-efficient

d) capable of evaluating the levels of one given inflammatory molecule in the context of multiple others

e) capable of performing repeated measures of the same cytokine panels in the same subjects under the same experimental assay condition

f) ability to reliably detect different proteins across a broad dynamic range of concentrations

While contemporary singleplex ELISA formats are able to accurately diagnose diseases whereby characterization of a single analyte is sufficient (i.e. HIV–1 p24); patient stratification and monitoring of more complex, multifactorial diseases such as cancers, graft-versus-host disease, autoimmune and neurodegenerative diseases require the analysis of multiple biomarkers in order to implement optimized therapeutic regimens. Accordingly, validation of novel biomarkers and their union into multiplex immunoassay panels confers the attractive prospect of simultaneous measurement of multiple biomarkers in a single patient sample, which reduces assay time, costs, and sample volume while concurrently enabling progression monitoring and outcome prediction to anticipate potentially severe sequelae or adverse drug reactions. Moreover, the advent of multiplex technology complements an ongoing progressive shift towards personalized medicine with holistic, molecular fingerprinting of diseases via the identification and characterization of biomarker profiles to accommodate greater diagnostic resolution between closely related disease phenotypes. For such reasons, it appears highly likely that multiplex immunoassays will continue to garner popularity and secure a mainstream role in research and eventually clinical spheres.

Multiple cytokines in a biological liquid sample can thus be recognized and measured by the differences in both bead sets, with chromogenic or fluorogenic emissions detected using flow cytometric analysis. Commercially available bead-conjugated antibodies allow the analysis of up to 25 different cytokines in one sample. However, this number can be expanded greatly if the scientist is willing to custom-conjugate antibodies of interest to one of nearly 100 different available beads. Multiplex ELISA are available from Quansys Biosciences (www.quansysbio.com) that coats multiple specific capture antibodies at multiple spots (one antibody at one spot) in the same well on a 96-well microplate. They provide Human Multiplex kits, mouse multiplex kits, Rat and Porcine multiplex kits and to support present-day research scenarios they also provide SARS COV2 multiplex kits. Q-Plex SARS Cov-2 4plex is ELISA for the detection of IgG antibodies to SARS Cov-2 in human plasma and serum. This kit is FDA approved. During this time of pandemic, it is unknown for how long antibodies persist following infection and if the presence of antibodies confers protective immunity. Hence this kit provides an advantage for the detection of the antibodies raised against infection


Q-Plex™ Human Angiogenesis (9-Plex)
Human Multiplex Kits

Ang-2, FGF, HGF, IL-8, PDGF-BB, TIMP-1, TIMP-2, TNFα, VEGF

Q-Plex™ Human Chemokine (4-Plex)

IL-8, IP-10, MCP-1, RANTES

Q-Plex™ Human Chemokine (9-Plex)

Eotaxin, GROa, I-309, IL-8, IP-10, MCP-1, MCP-2, RANTES, TARC

Q-Plex™ Human Cytokine – High Sensitivity (4-Plex)

IL-4, IL-6, IL-10, IFN-γ

Q-Plex™ Human Cytokine – High Sensitivity (15-Plex)

IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13, IL-15, IL-17, IL-23, IFN-γ, TNF-α, TNF-β

Q-Plex™ Human Cytokine – Inflammation (9-Plex)

IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IFNγ, TNFα

Q-Plex™ Human Cytokine Panel 1 (4-Plex)

IL-6, TNFα, IFNγ, IL-10

Q-Plex™ Human Cytokine Panel 2 (4-Plex)

IL-1α, IL-4, IL-6, TNFα

Q-Plex™ Human Cytokine Panel 1 (6-Plex)

IL-1β, IL-6, TNFα, IFN-γ, IL-1α, IL-10

Q-Plex™ Human Cytokine Panel 2 (6-Plex)

IL-6, IL-8, TNFα, IFN-γ, IL-4, IL-10

Q-Plex™ Human Cytokine – Screen (16-Plex)

IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-15, IL-17, IL-23, IFNγ, TNFα, TNFβ

Q-Plex™ Human Cytokine – Stripwells (16-Plex)

IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-15, IL-17, IL-23, IFNγ, TNFα, TNFβ

Q-Plex™ Human Cytokine Release Syndrome (16-Plex)

GMCSF, IFNα, IFNγ, IL-1β, IL-1RA, IL-2, IL-2Rα, IL-6, IL-6r, IL-8, IL-10, IL-12p70, IL-13, MCP-1, MIP-1α, TNFα

Q-Plex™ Human Female Hormone (8-Plex)

Adiponectin, Cortisol, C-Peptide, E1G, FSH, HCGb, IL-6, IL-10

Q-Plex™ Human Malaria (5-Plex)

HRP2, LDH-Pan, LDH-Pv, LDH-Pf, CRP

Q-Plex™ Human Micronutrient (7-Plex)

AGP, CRP, Ferritin, sTfR, RBP4, Tg, HRP2

Q-Plex™ Human MMP (6-Plex)

MMP-1, MMP-2, MMP-3, MMP-7, MMP-9 and MMP-13

Q-Plex™ SARS-CoV-2 Human IgG (4-Plex)

SARS-CoV-2 S1, SARS-CoV-2 S2, Sheep Fc (negative control), Anti-Human IgG (positive control)

Q-Plex™ SARS-CoV-2 Human IgG Quantitative (4-Plex)

SARS-CoV-2 S1, SARS-CoV-2 S2, Sheep Fc (negative control), Anti-Human IgG (positive control)

Mouse Multiplex Kits

Q-Plex™ Mouse Cytokine Inflammation (14-Plex)

IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-6, IL-10, IL-12p70, IL-17, MCP-1,TNFα, MIP-1α, GMCSF, RANTES

Q-Plex™ Mouse Cytokine Panel 1 (4-Plex)

MCP-1, MIP-1α, RANTES, TNFα

Q-Plex™ Mouse Cytokine Panel 2 (4-Plex)

IFN-γ, IL-1β, IL-6, TNFα

Q-Plex™ Mouse Cytokine Panel 3 (4-Plex)

IL-2, IL-4, IL-6, TNFα

Q-Plex™ Mouse Cytokine Panel 1 (6-Plex)

IFN-γ, IL-1α, IL-1β, IL-6, IL-10, TNFα

Q-Plex™ Mouse Cytokine Panel 2 (6-Plex)

IL-1β, IL-10, MCP-1, MIP-1α, RANTES, TNFα

Q-Plex™ Mouse Cytokine – Screen (16-Plex)

IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-17, MCP-1, IFN-γ, TNFα, MIP-1α, GMCSF, RANTES

Q-Plex™ Mouse Cytokine – Stripwells (16-Plex)

IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-17, MCP-1, IFNγ, TNFα, MIP-1α, GMCSF, RANTES

Rat and Porcine Multiplex Kits

Q-Plex™ Rat Cytokine – Inflammation (9-Plex)

IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12p70, IFN-γ, TNF-α

Q-Plex™ Porcine Cytokine – High Sensitivity (4-Plex)

IL-1β, IL-6, IL-8, TNFα