Product Description
| Cat Number | SRP-HM102 |
|---|---|
| Category | Recombinant Protein |
| Pack Size | 100UG |
| Description | Recombinant Human SFRP2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu25-Cys295. |
| Target | SFRP2 |
| Host | Human |
| Purity | > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
| Scientific Background | As biomarkers, DNA methylation is used to detect colorectal cancer (CRC) and make assessment of CRC prognosis. The published findings showed the association between the methylation of SFRP1, SFRP2, and WIF1, located in the Wnt signaling pathway, and the prognosis of CRC were not consistent. SFRP1, SFRP2, and WIF1 were frequently hypermethylated in CRC tumor tissues. It was apparent that the promoter hypermethylation of SFRP2 and co-hypermethylation of SFRP1 and SFRP2 might be considered as independent prognostic predictors for survival advantage of postoperative CRC patients. |
| Molecular Mass | The protein has a predicted MW of 32.4 kDa. Due to glycosylation, the protein migrates to 34-38 kDa based on Bis-Tris PAGE result. |
| Sl Sequence | Leu25-Cys295 |
| Solubility | Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
| Appearance | Lyophilized |
| Endotoxin Levels | Less than 1 EU per μg by the LAL method. |
| Expression System | HEK293 |
| Gene Id | Q96HF1 |
| Amino Acid Sequence | Recombinant Human SFRP2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu25-Cys295. |
| Storage | -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| View Document | |
| Note | The product is for research use only |
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