Human Siglec-5/CD170 Protein (SIG-HM205)
SKU: BTL-KB-P-03435 |
Host: Human |
Brand: Kactus Bio
Product Description
| Cat Number | SIG-HM205 |
|---|---|
| Category | Recombinant Protein |
| Pack Size | 100UG |
| Description | Recombinant Human Siglec-5/CD170 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Glu17-Thr434. |
| Target | Siglec-5/CD170 |
| Host | Human |
| Purity | > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
| Scientific Background | Interactions between endothelial selectins and the leukocyte counter-receptor PSGL1 mediates leukocyte recruitment to inflammation sites. PSGL1 is highly sialylated, making it a potential ligand for Siglec-5, a leukocyte-receptor that recognizes sialic acid structures. Binding assays using soluble Siglec-5 variants (sSiglec-5/C4BP and sSiglec-5/Fc) revealed a dose- and calcium-dependent binding to PSGL1. |
| Molecular Mass | The protein has a predicted MW of 73.1 kDa. Due to glycosylation, the protein migrates to 90-115 kDa based on Bis-Tris PAGE result. |
| Sl Sequence | Glu17-Thr434 |
| Solubility | Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
| Appearance | Lyophilized |
| Endotoxin Levels | Less than 1 EU per μg by the LAL method. |
| Expression System | HEK293 |
| Gene Id | O15389 |
| Amino Acid Sequence | Recombinant Human Siglec-5/CD170 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Glu17-Thr434. |
| Storage | -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| View Document | |
| Note | The product is for research use only |
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