ELISA Kit for Glucagon Like Peptide 1 Receptor (GLP1R)
SKU: BTL-CC-E-02620 |
Brand: Cloud Clone Corp
Product Description
| Cat Number | SED475Mu |
|---|---|
| Category | ELISA Kit |
| Pack Size | 96T, 96T×5, 96T×10, 96T×100 |
| Detection Method | Double-antibody Sandwich |
| Sensitivity | The minimum detectable dose of this kit is typically less than 0.055ng/mL |
| Detection Range | 0.156-10ng/mL |
| Reactivity | Mus musculus (Mouse) |
| Sample Type | tissue homogenates, cell lysates and other biological fluids |
| Assay Length | 3h |
| Research Area | Endocrinology; |
| Uniprot | O35659 |
| Target Synonyms | |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucagon Like Peptide 1 Receptor (GLP1R). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucagon Like Peptide 1 Receptor (GLP1R). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucagon Like Peptide 1 Receptor (GLP1R), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucagon Like Peptide 1 Receptor (GLP1R) in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
| Technical Manual | View Document |
| Note | The product is for research use only |
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