TransNGS® CUT&Tag Library Prep Kit for Illumina®
SKU: BTL-TB-MB-00452 |
Brand: Transgen Biotek
Product Description
| Cat Number | KP172-01 |
|---|---|
| Category | Molecular Biology Product |
| Pack Size | 12 rxns |
| Description | The kit is designed to prepare CUT&Tag libraries for mammalian cells for the Illumina high-throughput sequencing platform. The prepared libraries can be used for Single-end sequencing and Paired-end sequencing. Cleavage Under Target & Tagmentation (CUT&Tag) is a new method for studying protein interactions with DNA. Protein A/G-Tn5 transposase fusion protein and the target protein can form an immune complex through antibody bridging. Tn5 transposase is activated in the presence of Mg2+, and so short sequences containing adapters can be inserted into both ends of the DNA fragment that binds to the target protein. Finally, library can be constructed by PCR amplification. The kit is suitable for DNA research on the interaction of DNA-binding proteins such as transcription factors and co-regulators. Compared with traditional ChIP-seq, it has the advantages of more accurate and stable protein-DNA interaction information in low input of cells. Storage Box 1 is stored at 4°C for 1 year and Box 2 is stored at -20°C for 1 year Shipping Box 1 ice bag (4℃) and Box 2 Dry ice (-70℃) |
| Storage | -80°C |
| Note | The product is for research use only |
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