Product Description
| Cat Number | FM201-01 |
|---|---|
| Category | Molecular Biology Product |
| Pack Size | 10 rxns |
| Description | Fast MultiSite Mutagenesis System is used for generating mutated PCR fragments by introducing mutation sites on overlapping regions. High fidelity TransStart® FastPfu PCR SuperMix is included for amplification. This kit uses proprietary assembly mix and homologous recombination to seamlessly assemble up to six mutagenesis fragments. • Fast: Amplified with fast & high-fidelity 2×TransStart® FastPfu PCR SuperMix; only 15 minutes for recombination. • Flexible: Able to be cloned into any site to realize single-site/ multi-site, continuous/non-continuous mutagenesis. • Efficient: >90% mutagenesis efficiency. Notes We suggest performing 25 cycles for PCR. For low yield PCR products, we suggest using up to 30 cycles. Storage at -20°C for two years Shipping Dry ice (-70℃) |
| Storage | -80°C |
| References | 1 Li L, Fang C, Zhuang N, et al. Structural basis for transcription initiation by bacterial ECF σ factors[J]. Nature communications, 2019.(IF 11.90) 2 Deng L, Ren R, Liu Z, et al. Stabilizing heterochromatin by DGCR8 alleviates senescence and osteoarthritis[J]. Nature communications, 2019.(IF 10.70) 3 Wang Z, Wang S, Xu Z, et al. Highly promiscuous flavonoid 3-O-glycosyltransferase from Scutellaria baicalensis[J]. Organic letters, 2019.(IF 6.55) 4 Zhang L, Jiang D, Li Y, et al. Characterization of a novel type III alcohol dehydrogenase from Thermococcus barophilus Ch5[J]. International Journal of Biological Macromolecules, 2021.(IF 6.95) 5 Zhang L, Wang L, Wu L, et al. Biochemical characterization and mutational studies of a thermostable endonuclease III from Sulfolobus islandicus REY15A[J]. International Journal of Biological Macromolecules, 2021.(IF 6.95) |
| Note | The product is for research use only |
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