Product Description
| Cat Number | ELK8502 |
|---|---|
| Category | ELISA Kit |
| Pack Size | 1EA |
| Elisa Type | Competitive Inhibition |
| Sensitivity | 4.7 pg/mL |
| Standard | 1000 pg/mL |
| Detection Range | 15.63-1000 pg/mL |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length | 2.5h |
| Research Area | Endocrinology;Cardiovascular biology;Hepatology;Hormone metabolism; |
| Target Synonyms | Glucagon Like Peptide 1 |
| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat GLP1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GLP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GLP1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Standard Curve | View Document |
| Technical Manual | View Document |
| Product Url | View Document |
| Note | The product is for research use only |
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