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Human GLP1R(Glucagon Like Peptide 1 Receptor) ELISA Kit

SKU: BTL-ELK-E-05338 | Brand: ELK
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Product Description

Cat Number ELK4792
Category ELISA Kit
Pack Size 1EA
Elisa Type Sandwich
Sensitivity 0.056 ng/mL
Standard 10 ng/mL
Detection Range 0.16-10 ng/mL
Sample Type Tissue homogenates, cell lysates and other biological fluids
Assay Length 3.5h
Research Area Endocrinology;
Uniprot P43220
Target Synonyms GLP 1 R; GLP 1 receptor; GLP 1R; GLP; GLP-1 receptor; GLP-1-R; GLP-1R; GLP1R
Test Principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GLP1R. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GLP1R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GLP1R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GLP1R in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve View Document
Technical Manual View Document
Product Url View Document
Note The product is for research use only
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