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Human GLP-1 (Glucagon Like Peptide 1) ELISA Kit

SKU: BTL-EL-E-02850 | Brand: Elabscience
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Product Description

Cat Number E-EL-H6025_24T
Category Human ELISA Kit
Pack Size 24T
Detection Method Colormetric
Elisa Type Sandwich-ELISA
Sensitivity 0.94 pg/mL
Detection Range 1.56-100 pg/mL
Reactivity Human
Sample Type serum, plasma and other biological fluids
Assay Length 3 h 30 min
Research Area Cardiovascular, Neuroscience
Uniprot P01275
Target Synonyms GLP1
Test Principle This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GLP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GLP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GLP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GLP-1. You can calculate the concentration of Human GLP-1 in the samples by comparing the OD of the samples to the standard curve.
Storage 2-8℃/-20℃,12 months
References View Document
Standard Curve View Document
Technical Manual View Document
Msds View Document
Product Url View Document
Note The product is for research use only
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