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Human GLP-2 (Glucagon Like Peptide 2) ELISA Kit

SKU: BTL-EL-E-03163 | Brand: Elabscience
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Product Description

Cat Number E-EL-H2238_96T
Category Human ELISA Kit
Pack Size 96T
Detection Method Colormetric
Elisa Type Competitive-ELISA
Sensitivity 0.1 ng/mL
Detection Range 0.16-10 ng/mL
Reactivity Human
Sample Type serum, plasma and other biological fluids
Assay Length 2 h 30 min
Research Area Cancer; Metabolism; Developmental biology; Signal transduction; Stem cells
Uniprot P01275
Target Synonyms GLP2
Test Principle This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human GLP-2. During the reaction, Human GLP-2 in the sample or standard competes with a fixed amount of Human GLP-2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human GLP-2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human GLP-2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Storage 2-8℃/-20℃,12 months
References View Document
Standard Curve View Document
Technical Manual View Document
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Note The product is for research use only
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