pEASY®-Uni Seamless Cloning and Assembly Kit
SKU: BTL-TB-MB-00345 |
Brand: Transgen Biotek
Product Description
| Cat Number | CU101-03 |
|---|---|
| Category | Molecular Biology Product |
| Pack Size | 60 rxns |
| Description | This kit takes advantage of proprietary recombinases and homologous recombination to achieve directional recombination of PCR inserts with 15-25bp overlapping ends into any linearized vector, enabling efficient and seamless assembly of 1-7 fragments. • Fast: 5~15 minutes • Simple: No restriction enzyme digestions. • High efficiency: > 95% cloning efficiency. • Seamless: No extra sequences introduced. Storage Trans1-T1 Phage Resistant Chemically Competent Cells at -70°C for six months; others at -20°C for one year. Shipping Dry ice (-70℃) |
| Storage | -80°C |
| References | 1 Bai X, Sun P, Wang X, et al. Structure and dynamics of the EGFR/HER2 heterodimer[J]. Cell Discovery, 2023.(IF 38.07) 2 Shi C, Yang X, Hou Y, et al. USP15 promotes cGAS activation through deubiquitylation and liquid condensation[J]. Nucleic Acids Research, 2022(IF 14.90) 3 Jin Q, Yang X, Gou S, et al. Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression[J]. Science China Life Sciences, 2022.(IF 10.37) 4 Xu Y, Zhu T F. Mirror-image T7 transcription of chirally inverted ribosomal and functional RNAs[J]. Science, 2022.(IF 63.71) 5 Li B, Zhu L, Lu C, et al. circNDUFB2 inhibits non-small cell lung cancer progression via destabilizing IGF2BPs and activating anti-tumor immunity[J]. Nature communications, 2021.(IF 12.12) 6 Liu S, Fan L, Liu Z, et al. A Pd1–Ps–P1 feedback loop controls pubescence density in soybean[J]. Molecular plant, 2020.(IF 12.08) |
| Note | The product is for research use only |
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