Product Description
| Cat Number | CT501-02 |
|---|---|
| Category | Molecular Biology Product |
| Pack Size | 60 rxns |
| Description | pEASY®-T5 Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector are killed upon plating. Therefore, blue/white screening is not required. •5 minutes fast ligation of Taq-amplified PCR products. •High cloning efficiency. Positive clones up to 100%. •No blue/white selection needed. •Suitable for short and large fragment cloning. •Kanamycin and Ampicillin resistance genes for selection. •M13 forward primer and M13 reverse primer for sequencing. •T3 promoter and T7 promoter for in vitro transcription. •Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing. Storage Competent Cell at -70°C for six months; others at -20°C for 9 months Shipping Dry ice (-70℃) |
| Storage | -80°C |
| References | 1 Chen W, Chen L, Zhang X, et al. Convergent selection of a WD40 protein that enhances grain yield in maize and rice[J]. Science, 2022.(IF 63.71) 2 Zhao Z, Zhang Z, Li J, et al. Sustained TNF-α stimulation leads to transcriptional memory that greatly enhances signal sensitivity and robustness[J]. Elife, 2020.(IF 7.08) |
| Note | The product is for research use only |
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