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pEASY®-Blunt Simple Cloning Kit

SKU: BTL-TB-MB-00334 | Brand: Transgen Biotek
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Product Description

Cat Number CB111-02
Category Molecular Biology Product
Pack Size 60 rxns
Description pEASY®-Blunt Simple Cloning Vector eliminates the multi-cloning sites of pEASY® -Blunt Cloning Vector. It is designed for cloning and sequencing Pfu-amplified PCR products. •5 minutes fast ligation of Pfu-amplified PCR products. •Kanamycin and Ampicillin resistance genes for selection. •Easy blue/white selection. •SR primer and M13 forward primer for sequencing. •T7 promoter for in vitro transcription. •Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing. Storage Trans1-T1 Phage Resistant Chemically Competent Cell at -70°C for six months; others at -20°C for nine months Shipping Dry ice (-70℃)
Storage -80°C
References 1 Wu X, Yu C, Mu W, et al. The structural mechanism for transcription activation by Caulobacter crescentus GcrA[J]. Nucleic Acids Research, 2023.(IF 19.16) 2 Li Y, Du Y, Huai J, et al. The RNA helicase UAP56 and the E3 ubiquitin ligase COP1 coordinately regulate alternative splicing to repress photomorphogenesis in Arabidopsis[J]. The Plant Cell, 2022.(IF 12) 3 Wang X, Liu C, Zhang S, et al. N6-methyladenosine modification of MALAT1 promotes metastasis via reshaping nuclear speckles[J]. Developmental cell, 2021.(IF 10.09) 4 Wang H, Zhang L, Shang Y, et al. Emergence and evolution of highly pathogenic porcine epidemic diarrhea virus by natural recombination of a low pathogenic vaccine isolate and a highly pathogenic strain in the spike gene[J]. Virus evolution, 2020.(IF 5.50) 5 Li L, Fang C, Zhuang N, et al. Structural basis for transcription initiation by bacterial ECF σ factors[J]. Nature communications, 2019.(IF 11.90) 6 Zhang W, Wan H, Feng G, et al. SIRT6 deficiency results in developmental retardation in cynomolgus monkeys[J]. Nature, 2018.(IF 41.57)
Note The product is for research use only
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