TransScript® IV Reverse Transcriptase [M-MLV, RNase H-] (Anti Inhibition High Temperature RT)
SKU: BTL-TB-MB-00357 |
Brand: Transgen Biotek
Product Description
| Cat Number | AW101-02 |
|---|---|
| Category | Molecular Biology Product |
| Pack Size | 10000 units |
| Description | TransStart® IV Reverse Transcriptase is an M-MLV reverse transcriptase with high thermostability expressed and purified from E. coli after genetic modification. It enables a board range reaction temperature (42°C-65°C) and good resistance to inhibitors, with ultra-high thermal stability and deficient RNase H activity. Its high reaction temperature is beneficial to open RNA secondary structure when synthesizing the first strand cDNA, with the optimal reaction temperature of 50°C. It also has the characteristics of fast reaction speed, high sensitivity, high specificity, high yield (more full-length cDNA), and long half-life. Features Wide range of applications: severely degraded RNA can be used as a template for reverse transcription. Agnostic to inhibitors remaining in RNA. Fast reaction speed: only 10 minutes for reverse transcription. Strong synthesis ability: synthesis length up to 20kb. High thermal stability: reaction temperature 42°C-65°C. High sensitivity: high detection rate for trace RNA. Applications High-copy number and low-copy number gene detection. High GC or complex secondary structure RNA template. For difficult-to-handle RNA sample: degraded RNA template and RNA template containing RT enzyme inhibitor. cDNA library construction, primer extension, 3’ and 5’ RACE. Storage at -20 ℃ for two years Shipping Dry ice (-70 ℃) |
| Storage | -80°C |
| Note | The product is for research use only |
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