2×TransStart® GoldPfu PCR SuperMix (-dye)
SKU: BTL-TB-MB-00100 |
Brand: Transgen Biotek
Product Description
| Cat Number | AS401-02 |
|---|---|
| Category | Molecular Biology Product |
| Pack Size | 5×1 ml |
| Description | This product is a ready-to-use mixture of TransStart® GoldPfu DNA polymerase, dNTPs, and optimized reaction buffer showing high amplification efficiency, fast amplification, ultra high fidelity and high specificity. The SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and Nuclease-free Water. The amplified products with blunt ends can be directly cloned into pEASY®-Blunt vectors. The SuperMix without dye (-dye) cannot be directly loaded on agarose gel for electrophoresis. Its PCR product is not suitable for polyacrylamide gel electrophoresis. Reduced PCR operation time. Avoid contamination due to multi-step operation. 54-fold fidelity as compared to EasyTaq® DNA Polymerase. Amplification of genomic DNA fragment up to 15 kb. Amplification of plasmid DNA fragment up to 20 kb. Applications Ultra High fidelity PCR Site-directed mutagenesis Blunt end cloning Complex templates GC/ AT rich templates Long fragment PCR Storage at -20 ℃ for two years Shipping Dry ice (-70 ℃) |
| Storage | -80°C |
| Note | The product is for research use only |
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