| Description |
The kit contains a PerfectStart® Taq DNA Polymerase, optimized dual-cation buffer, SYBR Green I, dNTPs, PCR enhancer and PCR stabilizer. Three monoclonal antibodies bind to the hot-start Taq DNA Polymerase, effectively blocking DNA polymerase activity and inhibiting non-specific amplification at low temperature. qPCR SuperMix is provided at 2×concentration and can be used at 1×concentration by adding template, primers, Universal Passive Reference Dye and nuclease-free water.
Highlights
• Blocking by 3 antibodies; high specificity, sensitivity and amplification efficiency; applicable to a wide range of species.
• Dual-cation buffer enhances specificity and reduces primer-dimer formation.
• Universal Passive Reference Dye for different instruments to correct differences in fluorescence detection between wells due to pipetting errors.
Storage
at -20°C in dark for two years
Shipping
Dry ice (-70 ℃) |
| References |
1 Huang J, Wu C, Kloeber J A, et al. SLFN5-mediated chromatin dynamics sculpt higher-order DNA repair topology[J]. Molecular Cell, 2023.(IF 19.32)
2 Liang Y, Wang J, Xu C, et al. Remodeling Collagen Microenvironment in Liver Using a Biomimetic Nano‐Regulator for Reversal of Liver Fibrosis[J]. Advanced Science, 2023.(IF 17.52)
3 Cui B, Guo X, Zhou W, et al. Exercise alleviates neovascular age-related macular degeneration by inhibiting AIM2 inflammasome in myeloid cells[J]. Metabolism, 2023.(IF 13.93)
4 Deng P, Wang Z, Chen J, et al. RAD21 amplification epigenetically suppresses interferon signaling to promote immune evasion in ovarian cancer[J]. The Journal of Clinical Investigation, 2022.(IF 19.45)
5 He F, Cheng K, Qi J, et al. Black phosphorus nanosheets enhance differentiation of neural progenitor cells for improved treatment in spinal cord injury[J]. Chemical Engineering Journal, 2023.(IF 15.10)
6 Liang Y, Zhang J, Xu C, et al. Biomimetic Mineralized CRISPR/Cas RNA Nanoparticles for Efficient Tumor-Specific Multiplex Gene Editing[J]. ACS nano, 2023.(IF 17.10)
7 Xu J, Zhao J, Wang R, et al. Shh and Olig2 sequentially regulate oligodendrocyte differentiation from hiPSCs for the treatment of ischemic stroke[J]. Theranostics, 2022.(IF 11.55)
8 He F, Liu Z, Xu J, et al. Black phosphorus nanosheets suppress oxidative damage of stem cells for improved neurological recovery[J]. Chemical Engineering Journal, 2023.(IF 16.74)
9 Zhang J, Yuan J, Lin J, et al. Molecular basis of locus-specific H3K9 methylation catalyzed by SUVH6 in plants[J]. Proceedings of the National Academy of Sciences, 2023.(IF 12.77)
10 Li W, Ali T, Zheng C, et al. Anti-depressive-like behaviors of APN KO mice involve Trkb/BDNF signaling related neuroinflammatory changes[J]. Molecular Psychiatry, 2022.(IF 15.99)
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